Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papilloma virus type 16 E7 antibody (GTX133411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.